Zone de Texte: Results 2 (DAS-ELISA) Zone de Texte: Antibody dilutions/incubation conditions: coating: 1:250, 1:500, 1:1000 in carbonate buffer (pH 9.6) / 3 h at 37°C conjugate:1:250, 1:500, 1:1000 in PBS (pH 7.4)-tween 20 (0.05%)-PVP (2%)-albumin (0.2%) / 2, 3 h at 37°C Grinding: 1:4, 1:8 w/v in PBS -tween 20 (0.05%)-PVP (2%) + skimmed milk (2%, 5%) or in phosphate buffer (0.03 M) + DIECA (0.2% )

Previous
1  2  3  4  5  6  7  8  9  10  11  12 
Next