Zone de Texte: COMPLETE GENOME SEQUENCING OF CLAVIBACTER MICHIGANENSIS SUBSP. SEPEDONICUS Carol A. Isimaru1 , Julian Parkhill2 , David M. Francis3 , Susan E. Brown1 , Dennis L. Knudson1 1 Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523-1177, USA 2 Pathogen Sequencing Unit, Sanger Centre, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK 3 Department of Horticulture and Crop Science, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA ORGANISM STRAIN: Clavibacter michiganensis subspecies sepedonicus, strain ATCC 33113 BRIEF SUMMARY OF SEQUENCING STRATEGIES: The complete genome sequence will be generated at the Sanger Centre by a whole-genome shotgun strategy, generating 8-10 fold genome coverage from several libraries in pUC and m13. Sequencing reads will be produced using Big-dye terminators on ABI3700 capillary machines. End-sequences from large insert BAC and lambda libraries will also be generated, providing a scaffold to assemble the sequence contigs produced by the assembly of shotgun data. Gap closure will be achieved through a combination of primer walking, PCR, and shotgun strategies. The completed sequence will be analyzed and annotated using in house tools available at the Sanger Centre. DATA RELEASE PLAN: * A. Website Address(es): www.sanger.ac.uk/Projects/Microbes * B. Frequency of Data Release & Updates: according to Sanger Centre policy, periodic releases will be supplied to the NCBI for inclusion in their BLAST searches of unfinished genomes. TIMELINE FOR COMPLETION: * A. Start date: November 2001 * B. Anticipated completion date: October 2003 * C. Milestones: o Library Preparation and validation: 2 months from start date o Completion of random shotgun phase: 6 months from start date o Final Assembly: 6-12 months from start date o Annotation of sequence: 12-18 months from start date PLAN FOR MAINTENANCE & DISTRIBUTION OF REAGENTS: On completion and publication of the analysis and annotation, a fully annotated sequence will be released to the EMBL/GenBank/DDBJ public databases. PROJECT CONTACT: Name: Carol A. Ishimaru Phone: 970-491-5059 Fax: 970-491-3862 Email: Carol.Ishimaru@Colostate.edu

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