Zone de Texte: Positive: P. ramorum present Zone de Texte: Sample Zone de Texte: Isolation followed by morphological identification Zone de Texte: Conventional ITS-PCR Zone de Texte: Real-time ITS-PCR Zone de Texte: Negative: no P. ramorum present Zone de Texte: In case of uncertainty confirmation is recommended by one of the following methods: Conventional or real-time ITS-PCR ITS amplicon sequencing on a pure culture Cox PCR RFLP Zone de Texte: Positive: P. ramorum present Zone de Texte: Positive: P. ramorum present Zone de Texte: Negative: no P. ramorum present Zone de Texte: Positive Zone de Texte: Confirm by one of the following methods: Isolation followed by morphological identification Real-time ITS-PCR ITS amplicon sequencing on a pure culture Cox PCR RFLP Zone de Texte: ELISA Pre-screening Zone de Texte: Negative: No P. ramorum present Zone de Texte: Positive

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